Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Results of LEF1 and Notch2 immunohistochemical analysis in tumor tissue in relation to the clinicopathologic characteristics of CRC patients and their tumors.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Immunohistochemical expression of LEF1 and Notch2 in CRC and paratumorous normal colorectal tissues. (a) Immunohistochemical staining of LEF1 expression in paratumorous normal colorectal tissues (A1) and CRC tissues (A2–A4), (A1) negative expression (score 0), (A2) weak expression (score 1–4), (A3) moderate expression (score 5–8), and (A4) strong expression (score 9–12). (b) Immunohistochemical staining of Notch2 expression in paratumorous normal colorectal tissues (B1) and CRC tissues (B2–B4), (B1) strong expression (score 9–12), (B2) negative expression (score 0), (B3) weak expression (score 1–4), and (B4) moderate expression (score 5–8); scale bars, 25 μ m.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Results of LEF1 and Notch2 mRNA real-time PCR analysis in tumor tissue in relation to the clinicopathologic characteristics of CRC patients and their tumors.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Western blot analysis of LEF1 and Notch2 in CRC tissues and paratumorous normal colorectal tissues. β -actin was used as an internal control. (a) LEF1 expression in CRC tissues and the corresponding paratumorous normal colorectal tissues of the same patient. (b) Notch2 expression in CRC tissues and corresponding paratumorous normal colorectal tissues of the same patient. (c) The percentage of LEF1 and Notch2 positive specimens in CRC tissues (tumor) and corresponding paratumorous normal colorectal tissues (normal). χ 2 test was used for statistical analyses. ** P < 0.01.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Western Blot, Control, Expressing

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: A correlation between LEF1 immunohistochemical expression and Notch2 immunohistochemical expression in CRC. The dot figure showed a negative correlation between LEF1 expression and Notch2 expression (Spearman's rank test r = −0.315, P < 0.001). X -axis: Notch2 expression level of each patient; Y -axis: LEF1 expression level of each patient. Some of the data was overlapping. The size of the points reflected the number of data points in each location (i.e., larger points reflect a greater number of data points at a given location).

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Association of LEF1/Notch2 and clinical factors with overall survival.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques:

Journal: Disease markers

Article Title: Increased LEF1 Expression and Decreased Notch2 Expression Are Strong Predictors of Poor Outcomes in Colorectal Cancer Patients

doi: 10.1155/2013/983981

Figure Lengend Snippet: Kaplan-Meier survival curves showing significantly different survival rates for patients with CRC according to LEF1 expression and Notch2 expression. (a) Survival curves for LEF1; (b) Survival curves for Notch2; (c) survival curves for all patients divided by combination of LEF1 and Notch2 status; (d) survival curves for LEF1 high/Notch2 low and other combinations of LEF1/Notch2 expression.

Article Snippet: Slides were then incubated with blocking serum and primary antibodies for LEF1 (1 : 100, C12A5; Cell Signaling Technology, Danvers, MA) and Notch2 (1 : 200, D76A6; Cell Signaling Technology, Danvers, MA), overnight at 4°C.

Techniques: Expressing

Journal: Cell reports

Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases

doi: 10.1016/j.celrep.2019.05.061

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-LEF1 (C12A5) Al488 , Cell Signaling , Cat#8490S; RRID:AB_10949503.

Techniques: Virus, Plasmid Preparation, Recombinant, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Software, Extraction, Gene Expression